Antigenic analysis of Clostridium chauvoei flagella with protective and non-protective monoclonal antibodies

Production, characterization, and protective effect of monoclonal antibodies to Clostridium chauvoei flagella.

Monoclonal antibodies to flagella of Clostridium chauvoei were obtained by the fusion of murine myeloma cells (P3-X63-Ag8-U1) and spleen cells from BALB/c mice immunized with partially purified flagella of strain Okinawa. Enzyme-linked immunosorbent assay and Western blot analysis with partially purified flagella, flagellated cells, and nonflagellated mutants were used to show that five monoclo...

Antigenic analysis of Punta Toro virus and identification of protective determinants with monoclonal antibodies.

Hybridomas producing monoclonal antibodies to the three major structural proteins of Punta Toro virus (PTV) were established by fusion of spleen cells with Sp2/0-Ag-14 mouse plasmacytoma cells. Thirty-six independently derived monoclonal antibodies were evaluated in neutralization, hemagglutination inhibition, and ELISA assays and the isotype, antigen specificities, and cross-reactivities were ...

Protective Activity of Monoclonal Antibodies

The protective antigen of a highly immunogenic strain of clostridium chauvoei including an evaluation of its flagella as a protective antigen.

Evidence for Mitotic Crossing-over During the Parasexual Cycle of the Cellular Slime Mould Dictyostelium discoideum. By E. B. GINGOLD and J. M. ASHWORTH Growth of a Wild Strain and of a Pimelic Acid-utilizing Mutant of Pseudomonas azelaica on Aliphatic Dicarboxylic Acids. By L. JANOTA-BASSALIK and B. BOHDANOWICZ-STRUCI~~SKA . Plasmid-instability in acrA Mutants of Escherichia coli K12. By H. NA...

Immunizalion of Cattle with Clostridium chauvoei Vaccine